Canine Antibodies against Salivary Recombinant Proteins of Phlebotomus perniciosus: A Longitudinal Study in an Endemic Focus of Canine Leishmaniasis.

BACKGROUND

phlebotomine sand flies are vectors of Leishmania parasites. During a blood meal, sand flies deposit saliva into the skin of the host protein immunogenic obtain a specific antibody response. anti-saliva antibody allows the host to estimate exposure and sand flies, leishmaniasis in endemic areas, as well as the risk for Leishmania infection. 

However, the use of all salivary gland homogenates as antigens has some limitations, and therefore, recombinant salivary proteins have been tested to replace them in the antibody detection test. In this study, we have used for the first time sand fly saliva recombinant proteins in a longitudinal field study in dogs. Spinach Recombinant Proteins

RESULTS

Sera from dogs naturally exposed to the bites of P. perniciosus during two consecutive seasons of transmission in endemic sites for canine leishmaniasis (Canl) tested at different time points by ELISA for antibodies recognize the whole saliva, saliva single 43 kDa-related yellow recombinant proteins (rSP03B), and a combination of two recombinant proteins hard, yellow-linked 43 kDa and 35.5 kDa protein apyrase (rSP01). Dogs also tested positive for Leishmania infantum by serology, culture and PCR and infection status were prospectively evaluated. 

We found a significant association between infection and the number of active Canl anti-P. perniciosus saliva antibody. Importantly, we detected a high correlation between IgG antibody recognizes an antigen protein and salivary rSP03B whole. Kinetics of antibody response showed for both whole saliva and rSP03B same pattern is clearly associated with the seasonal abundance of P. perniciosus.

CONCLUSION

These results suggest that P. perniciosus rSP03B protein fish Recombinant Proteins is a valid alternative to whole saliva and can be used in large-scale serological studies. This new method could become practical and economical tool for detecting sound-host exposure to sand fly bites in endemic areas Canl.